Figure 6

PHA-4 is required for vulval transcription of lag-2. DR2429 daf-2(e1370); qIs56(unc-119 lag-2::gfp) animals were treated with control or pha-4 RNAi after hatching at 25°C until dauer larvae formed constitutively. The dauer larvae were then transferred to fresh RNAi plates and incubated at 15°C for 2 days until animals resumed development to the late L4 stage. Two out of four P6.p descendant cells with lag-2::gfp expression are indicated by arrow heads in a control DR2429 post-dauer L4 larva (A) and a DR2429 post-dauer L4 larva with pha-4 RNAi treatment (B). The other two P6.p descendant cells show similar lag-2::gfp expression patterns. The entire experiment was performed twice with 40 animals examined for each treatment. 37 out of 40 pha-4 RNAi-treated DR2429 post-dauer L4 larvae showed obviously diminished lag-2::gfp in four P6.p descendant cells compared to control animals. Anterior is left and dorsal is up. Scale bars, 10 μm.