Figure 9

Lbx2 is expressed in a segment-specific manner in the adult epididymis. Top panel: schematic representation of the five segments of the caput and the two regions of the corpus epididymis. Six μm paraffin sections of paraformaldehyde-fixed mouse epididymis were probed with a DIG-labeled antisense Lbx2 cRNA probe in in situ hybridization experiments. Lbx2 mRNA was detected by immunostaining using an alkaline phosphatase-coupled anti-DIG antibody (appears as a blue-purplish staining). Tissues were counterstained with Neutral Red to visualize nuclei. Caput (A, B, C) and corpus (D, E, F). (A) Localization of Lbx2 in segment 2 (s2) and 3 (s3) of the caput. The two segments are separated by a dotted line. (B) Magnification of segment 2 seen in A. (C) Magnification of segment 3 seen in A. (D) Lbx2 expression in the proximal (p) and distal (d) corpus. A dotted line separates the proximal and distal corpus. (E) Magnification of the proximal corpus seen in D. (F) Magnification of the distal corpus seen in (D). EE: epididymal epithelium; IT: interstitial compartment; LU: epididymal lumen. Magnifications: 100× (A, D); 200× (B, C, E, F).