Figure 8

Lbx2 is expressed in the principal and basal cells of the epididymal epithelium. Top panel: schematic representation of the caput epididymis and its 5 segments. A DIG-labeled antisense Lbx2 cRNA probe was used in in situ hybridization experiments on six μm paraffin sections of paraformaldehyde-fixed mouse adult epididymis. Lbx2 mRNA was detected by immunostaining using an alkaline phosphatase-coupled anti-DIG antibody (appears as a blue-purplish staining). Tissues were counterstained with Neutral Red to visualize nuclei. (A) 400× magnification of the segment 2 of the caput epididymis that reveals strong signal in the epididymal epithelium (EE). (B) The presence of Lbx2 can be observed in principal (arrow) and basal (arrowhead) cells using a 1000× magnification of (A). EE: epididymal epithelium; IT: interstitial compartment; LU; epididymal lumen.