Figure 4
Increase in number of cells undergoing proliferation. A-C: PCNA, E-cadherin and smooth muscle actin IHC shows that more cells undergo mitosis in the TG lobe mesenchyme and epithelium, and that a rim of smooth muscle cells surround the TG lobe. A: TG lung with non Fgf10FLAG expressing lobe ("WT") and transgene expressing lobe (TG). B and C are magnified from A. D-F: Phospho-histone H3, E-cadherin and smooth muscle actin IHC. D: TG lung with non Fgf10FLAG expressing lobe ("WT") and transgene expressing lobe (TG). Many cells are in M-phase in the TG lobe, but very few in the "WT" lobe. E and F are magnified from J.