Figure 4

PLCZ1 cRNA injection induces IP ₃ R-1 downregulation. a) Immunoblot. Five bovine oocytes were used per lane; samples were collected 12 hours after cRNA injection. MII oocytes were collected at the time of cRNA injection. Aged MII are noninjected oocytes that were left in culture the same amount of time as the injected ones. bPLCZ1 = bovine PLCZ1; mPLCZ1 = mouse PLCZ1. b) Quantification of relative abundance of IP₃R-1 versus the levels observed in MII oocytes. The number in the bars indicates relative IP₃R-1 mass. Data represented as mean ± SEM of two replications. Different letters indicate P < 0.06.