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Table 3 Relative histone H4 acetylation in five organs of dead cloned cattle compared to normal controla

From: Aberrant epigenetic changes and gene expression in cloned cattle dying around birth

 

Group

Heart

Liver

Spleen

Lung

Kidney

β-actin

N

1.00 ± 0.30

1.00 ± 0.17

1.00 ± 0.18

1.00 ± 0.12

1.00 ± 0.08

 

ED

0.82 ± 0.16

0.81 ± 0.32

1.02 ± 0.22

0.47 ± 0.15b

2.33 ± 0.13c

 

LD

1.26 ± 0.17

0.63 ± 0.15

0.61 ± 0.12b

0.82 ± 0.18

3.12 ± 0.79c

VEGF

N

1.00 ± 0.51

1.00 ± 0.31

1.00 ± 0.38

1.00 ± 0.23

1.00 ± 0.53

 

N

2.14 ± 0.09b

0.76 ± 0.19

1.07 ± 0.18

0.60 ± 0.04b

1.93 ± 0.59

 

LD

1.23 ± 0.16

0.47 ± 0.06b

1.27 ± 0.26

0.66 ± 0.08b

3.23 ± 0.49c

art2

N

1.00 ± 0.20

1.00 ± 0.54

1.00 ± 0.64

1.00 ± 0.40

1.00 ± 0.31

 

ED

0.49 ± 0.01c

5.54 ± 2.10b

2.88 ± 0.70c

4.49 ± 0.94c

0.47 ± 0.09b

 

LD

0.92 ± 0.12

3.53 ± 0.89b

0.35 ± 0.17

6.36 ± 1.03c

0.70 ± 0.11

oct4

N

1.00 ± 0.33

1.00 ± 0.60

1.00 ± 0.31

1.00 ± 0.44

1.00 ± 0.46

 

ED

1.36 ± 0.96

4.61 ± 0.71c

1.96 ± 0.52b

0.23 ± 0.09b

0.58 ± 0.19

 

LD

3.33 ± 0.40c

1.52 ± 0.14

1.12 ± 0.05

0.37 ± 0.17b

0.47 ± 0.27

TERT

N

1.00 ± 0.62

1.00 ± 0.68

1.00 ± 0.35

1.00 ± 0.37

1.00 ± 0.40

 

ED

1.70 ± 0.49

0.88 ± 0.32

1.74 ± 0.59

0.75 ± 0.19

0.51 ± 0.10b

 

LD

0.55 ± 0.27

0.63 ± 0.10

0.44 ± 0.02

0.81 ± 0.14

0.91 ± 0.15

H19

N

1.00 ± 0.37

1.00 ± 0.40

1.00 ± 0.19

1.00 ± 0.27

1.00 ± 0.49

 

ED

1.22 ± 0.40

1.78 ± 0.40b

2.92 ± 0.24c

0.73 ± 0.21

0.96 ± 0.40

 

LD

0.79 ± 0.16

0.56 ± 0.12

0.62 ± 0.18

0.62 ± 0.17

2.02 ± 1.01

Igf2

N

1.00 ± 0.08

1.00 ± 0.04

1.00 ± 0.62

1.00 ± 0.30

1.00 ± 0.27

 

ED

2.58 ± 0.84b

0.90 ± 0.20

1.16 ± 0.43

2.10 ± 0.47b

2.26 ± 0.80b

 

LD

1.07 ± 0.10

0.72 ± 0.21

0.37 ± 0.03

0.79 ± 0.05

1.37 ± 0.34

  1. a The relative histone H4 acetylation in two groups compared with normal control (the relative hiotone H4 acetylation of normal control were normalized to 1). Values are presented as the mean ± SEM. N denotes normal control, ED denotes early death group and LD denotes late death group.
  2. b (p < 0.05), c (p < 0.01) denote significant differences between ED and N, LD and N.