Figure 3
Addition of a selectable marker with pLoxP unc-119. (A) Map of pLoxP unc-119 showing the ampicillin resistance gene (ampR), R6K replication origin, and loxP site. (B) Integration of the pLoxP unc-119 plasmid into the fosmid carrying K10C2.4 as shown by PCR. The selected ampicillin-resistant colony (modified) carries both the unc-119 and R6K sequences from pLoxP unc-119 but absent in the parent fosmid which are detected via PCR with the indicated oligo sets. unc-119 unc-119 F and unc-119 R; R6K R6K F and R6K R.