Table 4 PCR primer for characterization of the insertion of the gene trap vector
Primer | Sequence (5' → 3') |
|---|---|
gene trap forward primer (genomic) | TGGGATGCAAACTCGGGTCCTA |
gene trap reverse primer (lacZ cassette) | CAAGGCGATTAAGTTGGGTAACG |
genotyping forward primer (wild type/mutant) | CAGGAACAGACTGAAACCATGC |
genotyping reverse primer (wild type) | AGGTTGTCAGACTGGCACTTCA |
genotyping reverse primer (mutant) | CACGCCATACAGTCCTCTTCAC |
ex 1_forward primer | CAGAGTGGGTCCGTAATCGT |
ex 7_reverse primer | TGCCCAGGATAAGTGGTTTC |
ex 8_reverse primer | ATTGCAGATGAAGTGGGTATCAG |
ex 8_forward primer | CCAGCCTGATACCCACTTCA |
ex 11_reverse primer | ATACCCGTTCCAATTCACTGTC |
ex 12_forward primer | CCAACAGTAGTAATTGGCTCAGGT |
ex 15_reverse primer | ACCTCGATCTCTGCTCTGAGTCT |