Table 2 Primers used for semi-quantitative RT-PCR and conditions of PCR reaction
From: RBM19 is essential for preimplantation development in the mouse
Gene | Dir | Primer sequences (5'-3') | Product size | Annealing temp (°C) |
|---|---|---|---|---|
P21 | F R | GTCCAATCCTGGTGATGTCC GTTTTCGGCCCTGAGATGT | 410 | 55 |
Bax | F R | TGCAGAGGATGATTGCTGAC ACTCCAGCCACAAAGATGGT | 313 | 55 |
CyclinG1 | F R | AATGTTGCTGGGAAGTCAGG GCAAGGTGCTGAGGTTTCTC | 352 | 55 |
Rbm19 | F R | CGGAGCGAAGTAAGACTGTG CTCTGTGGTGCTGAAGTTG | 470 | 53 |
β-geo | R | AGT ATC GGC CTC AGG AAG ATC G |  | 53 |
GAPDH | F R | ATTGTCAGCAATGCATCCTG TTCAGCTCTGGGATGACCTTGCC | 243 | 59 |