Figure 6
From: RBM19 is essential for preimplantation development in the mouse
Apoptosis and P53 activation in Rbm19GtXC768/GtXC768mutant embryos. Embryos from an Rbm19GtXC768/+ intercross were sorted based on appearance and designated either wild-type-appearing (Rbm19GtXC768/+ or Rbm19+/+) or Rbm19GtXC768/GtXC768mutants. (A) Detection of apoptosis by in situ TUNEL assay. Wild type-appearing blastocysts exhibit very few TUNEL-positive cells, whereas extensive apoptosis was observed in the growth-arrested Rbm19GtXC768/GtXC768embryos. (B) Cleaved caspase-3 staining of Rbm19GtXC768/GtXC768embryos showed strong staining of most cells in mutant embryos, suggesting apoptotic cell death. (C) P53 target gene expression in wild type appearing and Rbm19GtXC768/GtXC768mutant E3.5 embryos revealed by semi-quantitative RT-PCR. p21, Bax and CyclinG1 are upregulated in the mutant; GAPDH was used as a loading control.