Figure 8
ELP-1::GFP is associated with microtubules. ELP-1::GFP associates with fluorescent filaments in a squashed-worm preparation (A & D). A 1-minute treatment with levamisole (25 mM) and nocodazole (10 μM) shortens filaments (B & E). Longer treatments (2 minutes) eliminate fluorescent filaments (C & F). The boxes outlined with the dotted lines in A, B and C are enlarged in panels D, E, and F. Filaments in A & D were stable for at least 15 minutes of observation. The bright punctate fluorescence in the upper left of each panel is due to the autofluorescence of the gut granules (arrowhead). The spacing on the magnification bar is 10 μm. Similar results were observed in a total of three squashed-worm preparations.