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Figure 6 | BMC Developmental Biology

Figure 6

From: The human neonatal small intestine has the potential for arginine synthesis; developmental changes in the expression of arginine-synthesizing and -catabolizing enzymes

Figure 6

Expression of ASS, ARG and NOS in villus epithelium and neurons. Panel A: 40 μg of protein isolated from small-intestinal scrapings of a 60-year-old patient were loaded per lane (left column). Positive controls (right column) included 25 μg of human liver extract for ARG1, NOS1 and α-SMA, 25 μg of human kidney extract for ARG2, and 25 μg of mouse brain extract for NOS3. Absence of ARG1, NOS1 and NOS3 expression in the adult small-intestinal enterocytes is demonstrated. The density of the ARG2 band is approx. 10% of that in kidney. Panel B: Serial sections (5 μm) of the ileal myenteric plexus of a 6-year-old male child were stained for ASS, NOS1, and OAT. Note colocalization of ASS, NOS1, and OAT in intensely staining neurons (black arrows). Also note OAT-negative ganglia (white arrows). Another section of the same specimen was stained for ARG2. Scale bar: 100 μm.

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BMC Developmental Biology

ISSN: 1471-213X

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