Figure 5

Analysis of TCam-2 seminoma cell line. (A-B) Immunohistochemistry using the antibodies indicated. (C) Merge of (A) and (B). (D) Counterstaining with DAPI to detect nuclei. (E) RT-PCR cell lines TCam2 and JKT1 as well as Testis detecting expression of the indicated genes. (F) Western Blot of protein lysate from TCam2 cells detecting the proteins indicated. (G) Co-IP experiment using antibody to PRMT5 for IP and antibody to BLIMP-1 to detect potential interaction. – no Antibodyl; + IP using PRMT5 Antibody; Input Control. (H) Co-IP experiment using antibody to PRMT7 for IP and antibody to BLIMP-1 to detect potential interaction. – no Antibodyl; + IP using PRMT5 Antibody; Input Control. (I-M) Immunohistochemistry using the PRMT7 antibody (I), (K) Merge of (I) and (M), (L) Counterstaining with DAPI to detect nuclei, (M) brightfield image. Scale Bar indicates 25 μm.