An evolutionarily conserved intronic region controls the spatiotemporal expression of the transcription factor Sox10

Table 1 Spatial expression of GFP in sox10:GFP germline transgenic embryos is comparable to that deduced from transiently transgenic embryos

GFP expression was scored in detail from at least three embryos from each of the Tg(-4725sox10:GFP)^ba4and Tg(-1252sox10:GFP)^ba5lines. Embryos were examined at the stages indicated by fluorescent microscopy at 600× and cells expressing GFP scored by position and morphology. The fluorescent signal in each GFP expressing cell type was scored according to the following levels: – none observed, +/- very weak, + weak, ++ good, +++ strong, nd not determined. Data for the psox10^-1252:GFP construct was deduced from at least 40 transiently transgenic embryos.

ISSN: 1471-213X