Thyroid hormone components are expressed in three sequential waves during development of the chick retina

Trimarchi, Jeffrey M; Harpavat, Sanjiv; Billings, Nathan A; Cepko, Constance L

doi:10.1186/1471-213X-8-101

BMC Developmental Biology

Table 2 The kinetics of coexpression of TH components and marker genes at Stage 32

From: Thyroid hormone components are expressed in three sequential waves during development of the chick retina

gene 1	gene 2	% gene 1 ⁺	% gene 2 ⁺	% gene 1 ⁺ /gene 2 ⁺ *	% gene 2 ⁺ /gene 1 ⁺	% [ ³ H] ⁺	% of gene 1 ⁺ that are [ ³ H] ⁺	% of gene 2 ⁺ that are [ ³ H] ⁺	% of [ ³ H] ⁺ that are gene 1 ⁺	% of [ ³ H] ⁺ that are gene 2 ⁺
dio2	cath5	2.3 ± 0.5	9.4 ± 0.4	17.4 ± 4.5	4.0 ± 1.0	17.8 ± 0.9	0	2.0 ± 0.5	0	1.0 ± 0.3
dio2	TRb	3.2 ± 0.4	7.4 ± 1.1	14.3 ± 4.5	5.8 ± 1.3	16.4 ± 1.2	0	7.3 ± 0.9	0	3.3 ± 0.6
dio2	otx2	2.9 ± 0.1	30.5 ± 1.4	91.9 ± 4.2	8.7 ± 0.8	17.5 ± 1.0	3.2 ± 3.2	14.8 ± 0.3	0.5 ± 0.5	25.9 ± 1.8
dio2	dio3	2.9 ± 0.4	8.1 ± 0.9	0	0	16.4 ± 0.9	2.4 ± 1.2	42.8 ± 5.5	0.5 ± 0.2	20.8 ± 2.1
dio2	crx	3.3 ± 0.4	13.5 ± 1.0	70.9 ± 11.2	17.4 ± 3.3	17.8 ± 1.7	1.5 ± 1.5	0.6 ± 0.3	0.4 ± 0.4	0.5 ± 0.3
dio2	neurod	2.2 ± 0.2	23.6 ± 2.0	89.3 ± 2.2	8.5 ± 1.3	16.9 ± 1.1	2.1 ± 2.1	11.6 ± 1.1	0.3 ± 0.3	16.1 ± 1.0
TRb	neurod	8.7 ± 0.5	26.8 ± 0.6	95.5 ± 1.4	31.1 ± 1.7	17.9 ± 0.4	6.2 ± 1.3	12.1 ± 1.1	3.1 ± 0.9	18.1 ± 1.7
TRb	cath5	7.5 ± 0.9	12.5 ± 1.1	10.1 ± 2.6	5.9 ± 1.4	17.5 ± 0.6	10.1 ± 0.7	1.0 ± 0.6	4.2 ± 0.2	1.8 ± 0.6
TRb	otx2	7.7 ± 1.2	26.8 ± 0.7	95.7 ± 1.3	27.5 ± 3.9	17.5 ± 0.7	9.0 ± 2.6	14.3 ± 2.1	3.8 ± 0.8	21.6 ± 2.1
TRb	crx	6.6 ± 0.2	13.5 ± 0.7	67.5 ± 5.5	33.1 ± 2.9	17.3 ± 0.6	13.8 ± 3.5	1.8 ± 1.0	5.3 ± 1.3	1.4 ± 0.7
dio3	crx	10.7 ± 0.4	12.9 ± 1.6	0	0	18.9 ± 0.8	46.6 ± 1.4	1.0 ± 0.7	26.3 ± 0.6	0.6 ± 0.4
dio3	TRb	9.6 ± 0.9	8.1 ± 0.4	1.1 ± 0.6	1.1 ± 0.6	17.9 ± 1.1	50.9 ± 5.7	9.8 ± 0.9	26.8 ± 1.3	4.5 ± 0.9
dio3	neurod	8.5 ± 0.9	24.8 ± 3.0	24.8 ± 4.6	8.5 ± 1.3	18.1 ± 1.1	45.4 ± 6.0	8.3 ± 0.8	21.1 ± 1.7	11.7 ± 2.6
dio3	otx2	8.5 ± 1.0	25.8 ± 2.1	44.1 ± 13.4	13.4 ± 2.4	17.0 ± 0.9	45.3 ± 2.8	10.5 ± 1.7	22.4 ± 2.0	16.1 ± 3.3

Cells were labeled in ovo with [³H]-thymidine for 1 hour, and tissue was harvested and dissociated at the indicated times. Double DISH for the indicated genes and autoradiography for [³H] were carried out on the dissociated cells. Cells were quantified for labeling by a single gene probe, both gene probes, and labeling with [³H].
* % of cells that express gene 1 that also express gene 2

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ISSN: 1471-213X

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