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Table 2 The kinetics of coexpression of TH components and marker genes at Stage 32

From: Thyroid hormone components are expressed in three sequential waves during development of the chick retina

gene 1 gene 2 % gene 1 + % gene 2 + % gene 1 + /gene 2 + * % gene 2 + /gene 1 + % [ 3 H] + % of gene 1 + that are [ 3 H] + % of gene 2 + that are [ 3 H] + % of [ 3 H] + that are gene 1 + % of [ 3 H] + that are gene 2 +
dio2 cath5 2.3 ± 0.5 9.4 ± 0.4 17.4 ± 4.5 4.0 ± 1.0 17.8 ± 0.9 0 2.0 ± 0.5 0 1.0 ± 0.3
dio2 TRb 3.2 ± 0.4 7.4 ± 1.1 14.3 ± 4.5 5.8 ± 1.3 16.4 ± 1.2 0 7.3 ± 0.9 0 3.3 ± 0.6
dio2 otx2 2.9 ± 0.1 30.5 ± 1.4 91.9 ± 4.2 8.7 ± 0.8 17.5 ± 1.0 3.2 ± 3.2 14.8 ± 0.3 0.5 ± 0.5 25.9 ± 1.8
dio2 dio3 2.9 ± 0.4 8.1 ± 0.9 0 0 16.4 ± 0.9 2.4 ± 1.2 42.8 ± 5.5 0.5 ± 0.2 20.8 ± 2.1
dio2 crx 3.3 ± 0.4 13.5 ± 1.0 70.9 ± 11.2 17.4 ± 3.3 17.8 ± 1.7 1.5 ± 1.5 0.6 ± 0.3 0.4 ± 0.4 0.5 ± 0.3
dio2 neurod 2.2 ± 0.2 23.6 ± 2.0 89.3 ± 2.2 8.5 ± 1.3 16.9 ± 1.1 2.1 ± 2.1 11.6 ± 1.1 0.3 ± 0.3 16.1 ± 1.0
TRb neurod 8.7 ± 0.5 26.8 ± 0.6 95.5 ± 1.4 31.1 ± 1.7 17.9 ± 0.4 6.2 ± 1.3 12.1 ± 1.1 3.1 ± 0.9 18.1 ± 1.7
TRb cath5 7.5 ± 0.9 12.5 ± 1.1 10.1 ± 2.6 5.9 ± 1.4 17.5 ± 0.6 10.1 ± 0.7 1.0 ± 0.6 4.2 ± 0.2 1.8 ± 0.6
TRb otx2 7.7 ± 1.2 26.8 ± 0.7 95.7 ± 1.3 27.5 ± 3.9 17.5 ± 0.7 9.0 ± 2.6 14.3 ± 2.1 3.8 ± 0.8 21.6 ± 2.1
TRb crx 6.6 ± 0.2 13.5 ± 0.7 67.5 ± 5.5 33.1 ± 2.9 17.3 ± 0.6 13.8 ± 3.5 1.8 ± 1.0 5.3 ± 1.3 1.4 ± 0.7
dio3 crx 10.7 ± 0.4 12.9 ± 1.6 0 0 18.9 ± 0.8 46.6 ± 1.4 1.0 ± 0.7 26.3 ± 0.6 0.6 ± 0.4
dio3 TRb 9.6 ± 0.9 8.1 ± 0.4 1.1 ± 0.6 1.1 ± 0.6 17.9 ± 1.1 50.9 ± 5.7 9.8 ± 0.9 26.8 ± 1.3 4.5 ± 0.9
dio3 neurod 8.5 ± 0.9 24.8 ± 3.0 24.8 ± 4.6 8.5 ± 1.3 18.1 ± 1.1 45.4 ± 6.0 8.3 ± 0.8 21.1 ± 1.7 11.7 ± 2.6
dio3 otx2 8.5 ± 1.0 25.8 ± 2.1 44.1 ± 13.4 13.4 ± 2.4 17.0 ± 0.9 45.3 ± 2.8 10.5 ± 1.7 22.4 ± 2.0 16.1 ± 3.3
  1. Cells were labeled in ovo with [3H]-thymidine for 1 hour, and tissue was harvested and dissociated at the indicated times. Double DISH for the indicated genes and autoradiography for [3H] were carried out on the dissociated cells. Cells were quantified for labeling by a single gene probe, both gene probes, and labeling with [3H].
  2. * % of cells that express gene 1 that also express gene 2