Loss of replicating cells in transgenic fish expressing Dmrt1bY. A, B and C: Cell replication status was quantified at 80% epiboly (stage 17) by pH3 immunostaining. Wild type (A), CSKA:Dmrt1bY:GFP transgenic line. pH3 positive cells were counted for a constant given area (A, B) and revealed a highly significant (p < 0.001) decrease of 25.5% replicating cells in the transgenic line when compared to wild type (C). Scale bars: 120 μM.