Figure 1
From: Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY
Dmrt1bY overexpression modifies cell cycle pattern in cell culture as well as in live embryos. A and B: GFP fluorescence is cytoplasmic in mouse TM4 cells transiently transfected with p CMV:EGFP-SV40UTR control plasmid (A) while nuclear localized when transfected with pCMV:Dmrt1bY:GFP-SV40UTR construct (B). C and D: Radar histograms representing the DNA content distribution of Dmrt1bY:GFP relative to GFP (control) transfected cells expressed in percentage. "Ctr" and "GFP Ctr, GFP+" represent GFP negative and positive control cells in the plate tranfected with control GFP plasmid; similarly "Dmrt1bY, GFP+" and "Dmrt1b, GFP-" represent GFP negative and positive cells In the plate tranfected with Dmrt1bY:GFP plasmid. E and F: Cell cycle distribution reflected by DNA content in control versus stage 10 (MBT) and post-MBT (stage 13) Dmrt1bY-injected embryos.