Sema7A and plexinC1 are differentially expressed during rat neural development. Expression of Sema7A and plexinC1 during rat neural development was analyzed by Northern blot analysis (A) and RT-PCR (B). (A) Northern blot analysis of 40 μg E19 or adult (AD) rat whole-brain RNA was performed using Sema7A-specific probes. The blot was reprobed twice, first for plexinC1, and subsequently for GAPDH to control for the amount of RNA loaded in each lane. (B) E15 to adult rat whole-brain RNA was isolated and used for semiquantitative RT-PCR using Sema7A-, plexinC1- and GAPDH-specific primers. Experiments were done at least three times with similar results.