Comparative Bioinformatics Analysis of GATA-2 Genomic loci. (A) A syntenic arrangement of GATA-2 (solid black arrow) and RPN1 (diagonal striped arrow) is conserved between human, mouse, fugu and zebrafish showing that these regions are orthologous. By sequence comparison, three evolutionarily conserved elements were identified in the human, mouse, rat, fugu and zebrafish genomes, two upstream of GATA-2, located at 12.9 kbp (Up1, open arrowhead) and 10 kbp (Up2, solid arrowhead), and one downstream of GATA-2 (Down1, black dot). (B) Zebrafish GATA-2 locus has 6 exons (blocks). An open arrowhead indicates the location of Up1; a solid arrowhead indicates the locations of Up2 in zebrafish genomic sequence flanking GATA-2. Open blocks indicate the 5' UTR of zebrafish GATA-2; black blocks indicate zebrafish GATA-2 coding sequence. (C) Up1 stands for the genomic sequence lying 12892 bp to12811 bp upstream of the zebrafish GATA-2 start codon. The zebrafish Up1 sequence has an 81% sequence identity with the human Up1 sequence, an 80% sequence identity with both mouse and rat Up1sequences, and an 83% sequence identity with the fugu Up1 sequence. Up2 is the genomic sequence lying 10110 bp to 9887 bp upstream of the zebrafish GATA-2 start codon. Up2a is genomic sequence lying 10110 bp to 9999 bp upstream of GATA-2 start codon; Up2b is the genomic sequence lying 9998 bp to 9887 bp upstream of GATA-2. The zebrafish Up2 sequence has a 74%, 72%, 72% and 94% sequence identity with Up2 sequences in the human, mouse, rat and fugu genomes, respectively.