Gene expression studies of developing bovine longissimusmuscle from two different beef cattle breeds

Table 3 Examples of genes showing differential expression in longissimus muscle of bovine fetuses from different sire breeds in at least one time point¹

Gene²	GenBank Accession³	Number of array elements⁴	Gene expression ratio⁵ P60d/W60d	Gene expression ratio⁵ P135d/W135d	Gene expression ratio⁵ P195d/W195d	Gene expression ratio⁵ Pbirth/Wbirth
Aldolase A, fructose-biphosphate (ALDOA)	CF615006	4	0.87	1.04	2.16**	0.90
Collagens (COL1A1, COL1A2, COL3A1, COL12A1, COL15A1)	Additional file 2	42	1.21	0.70	0.96	0.64**
Cytochrome c oxidases (COX5B, 6C, 7B, 7C)	Additional file 2	9	0.93	0.99	0.84	1.74**
Fatty acid binding protein 4, adipocyte (FABP4)	CF614083	3	1.01	0.70**	0.90	0.98
Fatty acid binding protein 5 (FABP5)	CF613827	1	1.29	0.93	1.16	0.44**
Matrin 3 (MATR3)	CF614914	1	2.35**	1.15	0.79	0.64**
Ribosomal L proteins (RPL)	Additional file 2	32	0.75	0.93	1.01	1.52**
Ribosomal S proteins (RPS)	Additional file 2	13	0.80	0.89	0.99	1.51**

¹* indicates a statistically significant gene expression difference at P < 0.05 level; ** indicates a statistically significant gene expression difference at P < 0.01 level.
²Sequence annotation was carried out using approved gene symbols (human genome nomenclature) via the IBISS4 database [17]. BLAST [15] scores > 110 and e-values < 2 E-23 were accepted for annotation purposes.
³in cases where more than 1 microarray element was used to calculate the expression data, the accession number of 1 representative element is shown.
⁴Number of microarray elements representing the same gene that were used to calculate the expression data.
⁵To obtain the ratios shown, averaged absolute microarray signal intensity values for P (Piedmontese × Hereford) fetuses were divided by the signal intensities measured for W (Wagyu × Hereford) fetuses at d 60, d 135, d 195 and birth.

ISSN: 1471-213X