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Table 2 MS analysis of protein spot identified as GILT

From: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin

Observed peptide mass (Da, [M+H]+)1 Peptide sequence from MS/MS2 % confidence3 Matched database sequence4 E value5
1399.8 (CL)FNLVTELYK 100 (98) Observed 1
  CLFNLVTELYK 11
LFNLV + YK
1877
    Database 223
   SLFNLVCDTYK 233
 
1405.7 (TV)LDCVDGDLGNK 100 (90) Observed 1
   TVLDCVDGDLGNK 13
TVL+CV+GDLGNK
0.001
    Database 172
    TVLECVNGDLGNK 184
 
  1. MS analysis of the only detectable protein spot s2 (gray arrows in Fig. 9). 1Observed peptide masses resulting from the tryptic digestion of the protein spot s2, reported as singly charged. 2Peptide sequence information deduced from MS/MS spectra of the corresponding peptides from ESI-QqTOF analysis. The masses of isoleucine are indistinguishable from leucine in MS and therefore L can be I and vice versa. 3Percent confidence for the peptide sequences, as reported by PEAKS software for the ESI-QqTOF spectra. 4Highest homology match from protein database searching with the observed peptide sequences to X. tropicalis GILT. Indicates the sequence alignment of the observed peptide to the identified protein (Database) as aligned by BLASTp. The sequence in between indicates the matching and similar (+) amino acids between the two sequences.5From BLASTp alignments.