Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin

Table 2 MS analysis of protein spot identified as GILT

Observed peptide mass (Da, [M+H]⁺)¹	Peptide sequence from MS/MS²	% confidence³	Matched database sequence⁴	E value⁵
1399.8	(CL)FNLVTELYK	100 (98)	Observed 1 CLFNLVTELYK 11 LFNLV + YK	1877
			Database 223 SLFNLVCDTYK 233
1405.7	(TV)LDCVDGDLGNK	100 (90)	Observed 1 TVLDCVDGDLGNK 13 TVL+CV+GDLGNK	0.001
			Database 172 TVLECVNGDLGNK 184

MS analysis of the only detectable protein spot s2 (gray arrows in Fig. 9). ¹Observed peptide masses resulting from the tryptic digestion of the protein spot s2, reported as singly charged. ²Peptide sequence information deduced from MS/MS spectra of the corresponding peptides from ESI-QqTOF analysis. The masses of isoleucine are indistinguishable from leucine in MS and therefore L can be I and vice versa. ³Percent confidence for the peptide sequences, as reported by PEAKS software for the ESI-QqTOF spectra. ⁴Highest homology match from protein database searching with the observed peptide sequences to X. tropicalis GILT. Indicates the sequence alignment of the observed peptide to the identified protein (Database) as aligned by BLASTp. The sequence in between indicates the matching and similar (+) amino acids between the two sequences.⁵From BLASTp alignments.

ISSN: 1471-213X