Figure 6

RLK I cDNA and derived amino acid sequence and location of MS/MS peptide fragments. The complete nucleotide sequence (lower case) of RLK I cDNA is shown. Underlined nucleotide sequences indicate all the in-frame stop codons, the first methionine codon, and a consensus AATAAA polyadenylation signal. Numbers on the right indicate nucleotide position. Upper case letters indicate the deduced amino acid sequence (single letter code). Boxed sequences indicate tryptic peptides observed in the MS analyses of the RLK I protein spot from the 2D analysis. The underlined VEMDA sequence indicates a consensus caspase cleavage site identified in human type I keratins with the black inverted-triangle indicating the cleavage site. Black dots adjacent to two serine residues indicate possible phosphorylation sites based on those found in human K18 at Ser33 and Ser52 (here Ser34 and Ser52). Numbers on the left indicate amino acid position.