Skip to main content

Table 1 Morphological phenotypes of zebrafish embryos derived from fertilized eggs injected with different materials

From: Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo

Injected materials Concentration Number of embryos surviving among number of injected eggs Wild-type phenotype Abnormal Heart Development
gsk3α -MO 0.5 ng 146/155 (94.2%) 85 (58.2%) 61 (41.8%)
gsk3α -MO 1 ng 176/191 (92.1%) 61 (34.6%) 115 (65.4%)
gsk3α -MO 2 ng 212/273 (77.7%) 25 (11.8%) 187 (88.2%)
gsk3β -MO 2 ng 126/129 (97.7%) 88 (69.8%) 38 (30.2%)
gsk3β -MO 4 ng 137/144 (95.1%) 69 (50.4%) 68 (49.6%)
gsk3β -MO 6 ng 288/314 (91.7%) 36 (12.5%) 252 (87.5%)
axin1-MO 6 ng 151/164 (92.1%) 27 (17.9%) 124 (82.1%)
dsRed mRNA 100 pg 68/68 (98.8%) 83 (100%) 0 (0%)
Control MO 2 ng 105/108 (97.2%) 101 (96.2%) 4 (3.8%)
Control MO 6 ng 112/125 (89.6%) 103 (93.6%) 7 (6.4%)
gsk3α -mRNA_gsk3α -MO 50 pg + 2 ng 59/69 (85.5%) 18 (30.5%) 41 (69.5%)
gsk3α -mRNA_gsk3α -MO 100 pg + 2 ng 93/118 (78.8%) 51 (54.8%) 42 (45.2%)
gsk3β -mRNA_gsk3α -MO 50 pg + 2 ng 72/90 (80.0%) 9 (12.5%) 63 (87.5%)
gsk3β -mRNA_gsk3α -MO 100 pg + 2 ng 91/134 (67.2%) 5 (5.5%) 86 (94.5%)
gsk3β -mRNA_gsk3β -MO 50 pg + 6 ng 73/77 (94.8%) 45 (61.6%) 28 (38.4%)
gsk3β -mRNA_gsk3β -MO 100 pg + 6 ng 128/144 (89.0%) 96 (75.0%) 32 (25%)
gsk3α -mRNA_gsk3β -MO 50 pg + 6 ng 83/109 (76.1%) 15 (18.1%) 68 (81.9%)
gsk3α -mRNA_gsk3β -MO 100 pg + 6 ng 92/127 (72.4%) 8 (8.7%) 84 (91.3%)
dsRed-mRNA_gsk3α -MO 100 pg + 2 ng 83/96 (86.5%) 7 (8.4%) 76 (91.6%)
dsRed-mRNA_gsk3β -MO 100 pg + 6 ng 75/82 (91.4%) 13 (17.3%) 62 (82.7%)
  1. Fertilized eggs were injected at the 1-cell stage, and then gsk3α morphants were observed at 36 to 48 hpf; gsk3β morphants were observed the heart positioning at 24 to 36 hpf. Results are from three independent experiments. dsRed mRNA: served as a negative control.