Table 1 Morphological phenotypes of zebrafish embryos derived from fertilized eggs injected with different materials
From: Glycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo
Injected materials | Concentration | Number of embryos surviving among number of injected eggs | Wild-type phenotype | Abnormal Heart Development |
|---|---|---|---|---|
gsk3α -MO | 0.5 ng | 146/155 (94.2%) | 85 (58.2%) | 61 (41.8%) |
gsk3α -MO | 1 ng | 176/191 (92.1%) | 61 (34.6%) | 115 (65.4%) |
gsk3α -MO | 2 ng | 212/273 (77.7%) | 25 (11.8%) | 187 (88.2%) |
gsk3β -MO | 2 ng | 126/129 (97.7%) | 88 (69.8%) | 38 (30.2%) |
gsk3β -MO | 4 ng | 137/144 (95.1%) | 69 (50.4%) | 68 (49.6%) |
gsk3β -MO | 6 ng | 288/314 (91.7%) | 36 (12.5%) | 252 (87.5%) |
axin1-MO | 6 ng | 151/164 (92.1%) | 27 (17.9%) | 124 (82.1%) |
dsRed mRNA | 100 pg | 68/68 (98.8%) | 83 (100%) | 0 (0%) |
Control MO | 2 ng | 105/108 (97.2%) | 101 (96.2%) | 4 (3.8%) |
Control MO | 6 ng | 112/125 (89.6%) | 103 (93.6%) | 7 (6.4%) |
gsk3α -mRNA_gsk3α -MO | 50 pg + 2 ng | 59/69 (85.5%) | 18 (30.5%) | 41 (69.5%) |
gsk3α -mRNA_gsk3α -MO | 100 pg + 2 ng | 93/118 (78.8%) | 51 (54.8%) | 42 (45.2%) |
gsk3β -mRNA_gsk3α -MO | 50 pg + 2 ng | 72/90 (80.0%) | 9 (12.5%) | 63 (87.5%) |
gsk3β -mRNA_gsk3α -MO | 100 pg + 2 ng | 91/134 (67.2%) | 5 (5.5%) | 86 (94.5%) |
gsk3β -mRNA_gsk3β -MO | 50 pg + 6 ng | 73/77 (94.8%) | 45 (61.6%) | 28 (38.4%) |
gsk3β -mRNA_gsk3β -MO | 100 pg + 6 ng | 128/144 (89.0%) | 96 (75.0%) | 32 (25%) |
gsk3α -mRNA_gsk3β -MO | 50 pg + 6 ng | 83/109 (76.1%) | 15 (18.1%) | 68 (81.9%) |
gsk3α -mRNA_gsk3β -MO | 100 pg + 6 ng | 92/127 (72.4%) | 8 (8.7%) | 84 (91.3%) |
dsRed-mRNA_gsk3α -MO | 100 pg + 2 ng | 83/96 (86.5%) | 7 (8.4%) | 76 (91.6%) |
dsRed-mRNA_gsk3β -MO | 100 pg + 6 ng | 75/82 (91.4%) | 13 (17.3%) | 62 (82.7%) |