Injection of translation inhibitors gsk3α - and gsk3β -MO into embryos can specifically reduce the protein levels of GSK3α and GSK3β, respectively. The total protein lysate extracted from seven zebrafish embryos at 24 hpf was loaded on each lane and analyzed by western blot. The antibody used is indicated in the left of each blot. Anti-GSK3 antibody enables to recognize both GSK3α and GSK3β proteins; anti-α-tubulin antibody was used as a loading control. The protein levels of GSK3α and GSK3β were reduced greatly in the protein lysates extracted from the gsk3α – and gsk3β -MO-injected embryos, respectively.