Figure 9

Fluorescent in situ hybridization of MSX1 mRNA conducted with DIG labelled RNA antisense probe in bovine ovarian sections at day of estrus (A, B), day of ovulation (B, C), growth phase (D, E), dominance phase (F, G). Cytoplasms of the oocytes (Oo) are darkly stained with green fluorescent compared to cumulus cells (Cc). Negative controls were hybridized with DIG labelled RNA sense probe (H, I). Images from the same ovarian sections were captured with lower and higher magnification.