Figure 3

Quantification of TH expressing neurons derived from PA6-formed progenitors. Sox1-GFP expressing neural progenitors generated by co-culture with PA6 sorted at day 3–4 or day 7 and allowed to differentiate for a further 7 days under various conditions. No difference was observed in the number of neurons that expressed TH in unsorted control cultures, reconstituted cultures, sorted GFP^pos cells re-placed onto PA6 or onto PDL-Laminin coated plastic (A) or when plated back onto different coatings or feeder cells (B). Likewise, no difference was found in the generation of TH expressing neurons between neural progenitors sorted at day 3–4 and day 7(C).