Figure 2

Flies with mutations in CG2904 have rough eyes, defects in IOC sorting, an increase in IOC number (A-F) SEM views of adult fly eyes of various genotypes. (G-O) Pupal retinas of various genotypes stained with anti-Dlg. (A, G) Wildtype flies have regularly spaced ommatidia and an invariant number of IOCs. Cell types indicated are bristle (B), 2°, 3°, and asterisk represent extra IOCs. (B,H) ec¹ flies obtained from the Bloomington Stock center have rough eyes and a modest number of extra 2° and 3° pigment cells. (C,I) GMR-driven RNAi of CG2904 results in flies with rough eyes and a large increase in IOCs, with many stacked side-by-side in parallel rows. (D,J) Flies homozygous for a deletion in CG2904, ec^EPΔ4, have rough eyes, a large increase in IOCs, with many cells stacked side-by-side in parallel rows. (E,K) GMR-dependent expression of ec-SF1 has no effect on the adult eye and does not cause any excess death of IOCs. (F,L) Expression of GMR-ec-SF1 restores normal levels of IOC death to ec^EPΔ4 flies. (M,N) Pupal eyes from two independent stocks of ec¹ outcrossed for 5 generations. There are increased numbers of IOCs as compared with the original ec¹ stock, and many extra cells are aligned side-by-side in parallel rows. (O) Pupal eyes from ec^3c3 flies have a modest increase in IOC number and few defects in cell sorting.