Figure 6

A: Compared evolution of cell production obtained from GFP⁺ cell sorted from 8 dpc YS infected upon explantation (between bracket: number of cells recovered at day 8), or after one day in organ culture. B: Evolution of GFP expression in the whole population or in GFP^- cells sorted from transduced 8 dpc OrgD1-YS after culture on OP9 stromal cells. A steady state GFP expression is attained at day 4 post-infection. Moreover, GFP expression is acquired, during culture, by a subset of GFP^- sorted cells. C: GFP expression in transduced OrgD1-YS cells (top panel left) persists in sorted GFP⁺ cells cultured on OP9 stromal cells (top panel right). The colonies generated by transduced cells also remain entirely GFP⁺ (bottom panel).