Table 4 List of primers for semi-quantitative RT-PCR

1

carboxypeptidase A1

CarbA1F2 5'-gcattatcttgattcgggtc-3' CarbA1RT 5'-catcttgcccacagtttc-3'

2

cathepsin L

CathLF1 5'-gcggaatacggacaatacac-3' CathLR2 5'-tagtgattagcgtggtcgcg-3'

3

lipopolysaccharide binding protein

LipoF2 5'-acttcgctgcagtcttcaag-3' LipoR2 5'-cgtcgtgcctgtgctattgc-3'

4

immune reactive putative protease inhibitor

InhibiF1 5'-tttgagttgcgttgtggtgg-3' InhibiR2 5'-tcaccaacaggtatgcattc-3'

5

lipase

LipaseF2 5'-aggatttgggcgatttcatc-3' LipaseR2 5'-ttggtcaagcagcctggttg-3'

6

trypsinogen Y precursor

TrypF2 5'-ggttggggaagtcgaaactc-3' TrypR2 5'-ctcaggaaaagctctaacag-3'

7

conserved hypothetical protein [Lactococcus lactis]

LactF2 5'-ttagttaccaagattggctc-3' LactR2 5'-gaatgtcaaaccacttgtgc-3'

8

titin-like protein

TitinF1 5'-gaggaaatcgttgacgacac-3' TitinR2 5'-tttctaccttcggacctacc-3'

9

Na-K-ATPase

ATPaseF2 5'-atactgaggacctgccttcc-3' ATPaseR2 5'-actggcccgatgttctccac-3'

10

hmg176

HMG176F 5'-atgaaaagtttccttgtcatc-3' HMG176R 5'-cttaatctaaccaagaaaccac-3'

11

carboxypeptidase A2

CarbA2F2 5'-ccagaatctaaagcaattgc-3' CarbA25-P 5'-aaagccttaacggccagtgtc-3'

12

tissue plasminogen activator

tPAF2 5'-tggtatcaacgcagagtagc-3' tPAR3 5'-atgagagtatttgcccgtgg-3'

13

thymosin beta homologue

ThymoF1 5'-ggaacccaaaccggttcaag-3' ThymoR1 5'-attcagtcttgtcagcgacc-3'

14

TNF receptor homolog 3

TNFF1 5'-aagtttccttgtcatcgccc-3' TNFR1 5'-ctactagaagctcctgtgtc-3'

15

unknown EST

UNF1 5'-aacatgtcccagcggctaac-3' UNR1 5'-tcaacctgtcagcgtgacag-3'

16

ribosome protein S23

S23F 5'-ggtacgcgggattgaagagaacg-3' S23R 5'-caacacagctttgcagcagcagg-3'

17

ribosome protein L27

L27F 5'-cctcagacaagccctacggac-3' L27R 5'-ctcttgtatctctcctcgaag-3'

18

ribosome protein S7

S7F 5'-catccggatcaagctcgacgg-3' S7R 5'-ttcctaagtttacaagtaggg-3'

19

ribosome protein L11

L11F 5'-catctgtgtgggagagtctggtg-3' L11R 5' – gtgttgtcagtctcactcgctgc-3'

20

G-protein-γ

Gproγ 5'-atggatatgatggtatcaacg-3' Gproγ 5'-ttaaagaacagtgcaggaact-3'

21

NTF-2

NTF2F 5'-atggcgctcaatccacaatac-3' NTF2R 5'-ctttgaaggagtacagttgca-3'

22

ecdysteroid-regulated gene

EcdyrF 5'-ctgcaggatgccgtcatac-3' EcdyrR 5'-ctaggcgcgcggaggagcgat-3'

23

beta-actin

ActinF 5'-agtagccgccctggttgtagac-3' ActinR 5'-ttctccatgtcgtcccagt-3'