Table 1 Identification of putative genes from SSH of tester cDNA from molting 5th instar larvae and driver cDNA from feeding 6th instar larvae
Classification | No | Tissue Sources | Similar genes in GenBank | E-value | Functions of the genes |
|---|---|---|---|---|---|
Ribosome proteins | 1 | head midgut epidermis | ribosomal protein L27 [S. frugiperda] | 1e-55 | participates in both 50 S subunit assembly and the peptidyl transferase reaction |
| Â | 2 | head | ribosomal protein S7 [S.frugiperda] | 7e-23 | initiating assembly of the head of the 30S subunit |
| Â | 3 | fat body midgut | ribosomal protein S2 [B. mori] | 2e-40 | maintaining balance between the small and large ribosomal subunits |
| Â | 4 | midgut | ribosomal protein L17/23 [Apis mellifera] | 8e-17 | regulator of the p53-MDM2 feedback regulation |
| Â | 5 | midgut | ribosomal protein L38 [Plutella xylostella] | 2e-17 | protein of 60 S ribosomal subunit |
| Â | 6 | haemocyte | ribosomal protein S24 [B. mori] | 1e-21 | protein of 40 S ribosomal subunit |
| Â | 7 | head | ribosomal protein L22 [S. frugiperda] | 2e-29 | large ribosomal subunit |
| Â | 8 | midgut | ribosomal protein L13 [S. frugiperda] | 3e-43 | 60 S ribosomal subunit protein |
Hydrolases | 9 | fat body | molting fluid carboxypeptidase A [B. mori]-1 | 3e-31 | pupal ecdysis, recycling of the amino acids |
| Â | 10 | haemocyte | cathepsin L like protease [Glossina morsitans morsitans] | 1e-25 | larval molting and cuticle and eggshell remodeling |
| Â | 11 | midgut | trypsinogen Y precursor [Gadus morhua] | 2e-06 | hydrolase |
| Â | 12 | epidermis | molting fluid carboxypeptidase A [B. mori]-2 | 1e-54 | molting-related |
| Â | 13 | epidermis | plasminogen activator, tissue [Mus musculus] | 7e-07 | hydrolase |
| Â | 14 | midgut | lipase-1 [B. mandarina] | 2e-23 | lipid digestion |
| Â | 15 | fatbody | chitinase [H. armigera] (BLAST by nucleotide acids) | 0.42 | hydrolase |
Immune-related Genes | 16 | haemocyte | lipopolysaccharide binding protein [B. mori] | 6e-26 | immune |
| Â | 17 | fat body | immune reactive putative protease inhibitor PrInh6 [Glossinamorsitans morsitans] | 2e-09 | immune |
Regulators | 18 | fat body haemocyte | CHK1 checkpoint homolog [Xenopus tropicalis] | 2e-07 | DNA damage and replication checkpoints |
| Â | 19 | midgut | CG13323-PA [D. melanogaster] (similar to hmg176) | 6e-05 | - |
| Â | 20 | epidermis | thymosin beta homologue [Ciona intestinalis] | 0.55 | actin-remodeling, wound healing, angiogenesis |
enzyme | 21 | head | Na-K-ATPase [Anopheles gambiae] | 2e-09 | enzyme |
Others | 22 | midgut | TNF receptor homolog 3 [Rattus norvegicus] | 3 | cellular proliferation, differentiation and programmed cell death |
| Â | 23 | epidermis | Titin-like protein [B. mori] | 8e-15 | roles in chromosomes and muscles |
| Â | 24 | midgut | Acyla-CoA binding protein [B. mori] | 4e-06 | intracellular acyl-CoA transporters |
| Â | 25 | fat body | hypothetical protein Llacc01000286 [Lactococcus lactis subsp.cremoris SK11] | 1e-24 | - |
| Â | 26 | head | CG32972-PB, isoform B [D.melanogaster] | 0.011 | - |
| Â | 27 | fat body | 16S rRNA [S. frugiperda] (BLAST by nucleotide acids) | 7e-17 | - |
Unknown ESTs | 28–35 | epidermis | Unknown proteins |  | - |