Figure 8

Myt1 can partially rescue the inhibitory effect by Notch1^ICD on Ngn3 function. (A-K). Immunohistochemical stainings (A, E, I-K) and in situ hybridizations (B-D, F-H) on sections in the duodenum 52 hours after electroporation with Ngn3 (A-D, I), Ngn3 and Notch1^ICD (E-H, J) or Ngn3 with Notch1^ICD and MyT1 (K) showing expression of Ngn3 (A, E), NeuroD (B, F), Myt1 (C, G), Hes6-1 (D, H), and βIII-tubulin. Note that Ngn3 fails to induce expression of endocrine markers in the presence of Notch1ICD. βIII-tubulin expression is partially restored when Notch1^ICD and MyT1 are co-expressed with Ngn3 (compare K to J and I).