Figure 7

Differentiation can be uncoupled from delamination. (A-H). Confocal sections of chicken embryonic pancreata 52 hours after electroporation with Ngn3 (A-B), Ngn3 and Notch1^ICD (C-D), NeuroD (E-F), or NeuroD and Notch1^ICD (G-H) subjected to immunohistochemical stainings against Pax6 (red) and laminin (blue) (A, C, E, G) and glucagon (red) and somatostatin (blue) (B, D, F, H). GFP was detected by its endogenous fluorescence (A-H). Inserts show higher magnification of boxed areas in the cognate panels. Note that Notch1^ICD inhibits Ngn3 and NeuroD induced expression of endocrine markers without affecting delamination of the cells from the endoderm.