Figure 3

Cell autonomous effects of ligand expression is reversed by Notch1^ICD. 3D projections of confocal optical sections of chicken embryos subjected to whole mount immunohistochemical stainings for GFP (green) and glucagon plus insulin (red) (A-C) or GFP (green), glucagon (red) and amylase (blue) (D, E) 72 hours after electroporation with the indicated constructs. The most extreme phenotype resulting from ectopic Delta1 expression is an almost complete conversion of the pancreas into a tubular structure composed almost entirely of endocrine cells (compare B to A). When Delta1 is co-expressed with Notch1^ICD most transfected cells do not express the hormones (C). D and E show the entire dorsal pancreas at the same magnification (scale bar represents 100 μm). Note the even distribution of electroporated cells in the control (D) and the clustering of electroporated cells in the endocrine population in the Delta1 electroporated pancreas.