Figure 3
From: Generation of FGF reporter transgenic zebrafish and their utility in chemical screens
D2EGFP expression is dependant on FGF signaling. (A & B) Lateral views of gastrula staged Tg(Dusp6:d2EGFP)pt6 embryos. Control uninjected is shown in (A), while in (B) fgf8 mRNA injected at the 2-cell stage. Expression of d2EGFP is greatly expanded in fgf8-injected embryos. (C & D) Lateral views of 28 hpf transgenic embryos. (C) Control MO injected (D) fgf8-MO injected embryo shows loss of d2EGFP expression within the MHB, dorsal retina and smaller otic vesicles. Red arrowhead: MHB; yellow arrow: dorsal retinal; blue arrowhead: optic stalk; red bracket: otic vesicle.