Table 3 List of the primer pairs for quantitative RT-PCR. Twenty-three genes were selected as being up-regulated in stage X embryo from microarray data
From: A set of stage-specific gene transcripts identified in EK stage X and HH stage 3 chick embryos
# | GeneChip Probe Set ID | Gene Title | Forward | Reverse |
|---|---|---|---|---|
1 | Gga.11040.1.S1_at | similar to hypothetical protein BC009518 | tgctggtcaacttatgccac | gcactggaactgaaatgctg |
2 | Gga.12503.2.S1_at | Nucleoredoxin | gcagaacaaggtggtggg | gtggtcggaggagatgaaga |
3 | Gga.13029.1.S1_at | Finished cDNA, clone ChEST43l14 | tgcccttcatacctcctgac | catttattcttccaccccca |
4 | Gga.14754.1.S1_at | death associated transcription factor 1 | gacaaagcagcagagcacac | gcacccaaactggaagatg |
5 | Gga.16489.1.S1_at | Finished cDNA, clone ChEST769a21 | gaatcggtgtgtgacttggtt | tctccaacttccacagcaca |
6 | Gga.17114.2.S1_a_at | Finished cDNA, clone ChEST485a9 | gtttcccttcccaacaggag | aaaagcccctctgattcca |
7 | Gga.8833.1.S1_at | Transcribed locus | agggcggctttttcagatac | ttgctttttgctctcctcatc |
8 | GgaAffx.10043.1.S1_at | similar to Topoisomerase I binding, arginine/serine-rich | catcttccttcctggggact | ctgcctgtgcttcttgactg |
9 | GgaAffx.11175.1.S1_at | similar to 12-transmembrane domain co-transporter Ce11 | agccttcagagagcagcaac | ttggagaacacaaagacccc |
10 | GgaAffx.11653.1.S1_s_at | serine/threonine kinase 17b (apoptosis-inducing) | atgcttgaacagaaaacggg | ttgaacttagaaagggggca |
11 | GgaAffx.21832.1.S1_s_at | fibroblast growth factor 13 | acagcaggcaaggataccac | aagacccaaataccaacccc |
12 | GgaAffx.2367.1.S1_at | NA | gaacctgtaaccccgaatga | ctttccttttccgatttccc |
13 | GgaAffx.4101.1.S1_at | similar to transmembrane protein 20 | tgattctcctctactatgctttcca | tcccaaacgctgtatttttctt |
14 | GgaAffx.4973.2.S1_s_at | similar to TNF receptor-associated factor 6 | atttgagcctgccctcttct | tgtgagtgttttgcgtctcc |
15 | GgaAffx.5963.1.S1_at | similar to RIKEN cDNA C030048B08 | tccaagaaaccagggagaag | tgactgtgattgccagaagg |
16 | GgaAffx.8887.1.S1_s_at | similar to VANIN 3 | gaagggaaactggtggctc | cagaaagggcaagacattca |
17 | GgaAffx.9105.1.S1_at | similar to Stromal interaction molecule 2 precursor | gggtagttatgccccgagtt | ccagtgtgcttggaggtttt |
18 | GgaAffx.9296.3.S1_s_at | similar to RNA polymerase II elongation factor ELL2 | accaccagcaccagtcattt | gcttcttgttcatcagggga |
19 | Gga.17330.1.S1_at | splicing factor, arginine/serine-rich 15 | ggagccctctcaagtcactg | tgaccggagactgagctttt |
20 | Gga.318.1.S1_at | potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2 | catcttgctcggactcatca | gcgtaagaaaaggcaagtcg |
21 | Gga.4401.1.S1_a_at | prostaglandin-endoperoxide synthase 2 | tgcaacgatatggctgagag | gggtgccagtggtacagagt |
22 | GgaAffx.20623.1.S1_s_at | LOC420789 | tgttgaagcatacttccctt | cttgcctcttcatttgattc |
23 | GgaAffx.7728.1.S1_at | similar to velo1 | accagtcccagagaacatgg | gtgattttgcccgttgactt |