Table 1 Primer details used for qRT-PCR
Gene | Genbank Accession number | Sequence | Ta (°C) | amplicon size (bp) |
|---|---|---|---|---|
B2M | NM_213978 | 5'-TTCACACCGCTCCAGTAG-3' 5'-CCAGATACATAGCAGTTCAGG-3' | 59.5 | 166 |
BACT | AY550069 | 5'-CATCACCATCGGCAACGAGC-3' 5'-TAGAGGTCCTTGCGGATGTC-3' | 55.8 | 141 |
GAPDH | AF017079 | 5'-TCGGAGTGAACGGATTTG-3' 5'-CCTGGAAGATGGTGATGG-3' | 51.1 | 219 |
H2A | BX921568 | 5'-GCTGTTGGGCAAAGTCAC-3' 5'-GGCTCTCCGTCTTCTTGG-3' | 54.3 | 83 |
PGK1 | AY677198 | 5'-AGATAACGAACAACCAGAGG-3' 5'-TGTCAGGCATAGGGATACC-3' | 56.4 | 126 |
S18 | NR_002170 | 5'-GGCTACCACATCCAAGGAAG-3' 5'-TCCAATGGATCCTCGCGGAA-3' | 58.7 | 149 |
UBC | M18159 | 5'-TTCGTGAAGACCTTGACTG-3' 5'-GGACTCCTTCTGGATGTTG-3' | 51.1 | 186 |