Skip to main content

Table 1 Primer details used for qRT-PCR

From: Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos

Gene Genbank Accession number Sequence Ta (°C) amplicon size (bp)
B2M NM_213978 5'-TTCACACCGCTCCAGTAG-3'
5'-CCAGATACATAGCAGTTCAGG-3'
59.5 166
BACT AY550069 5'-CATCACCATCGGCAACGAGC-3'
5'-TAGAGGTCCTTGCGGATGTC-3'
55.8 141
GAPDH AF017079 5'-TCGGAGTGAACGGATTTG-3'
5'-CCTGGAAGATGGTGATGG-3'
51.1 219
H2A BX921568 5'-GCTGTTGGGCAAAGTCAC-3'
5'-GGCTCTCCGTCTTCTTGG-3'
54.3 83
PGK1 AY677198 5'-AGATAACGAACAACCAGAGG-3'
5'-TGTCAGGCATAGGGATACC-3'
56.4 126
S18 NR_002170 5'-GGCTACCACATCCAAGGAAG-3'
5'-TCCAATGGATCCTCGCGGAA-3'
58.7 149
UBC M18159 5'-TTCGTGAAGACCTTGACTG-3'
5'-GGACTCCTTCTGGATGTTG-3'
51.1 186
  1. Ta = optimal annealing temperature