Figure 8

Expression after chemical ablation. In all panels, the left half corresponds to the unablated side and the right half corresponds to the ablated side. Panels A, C, E, G, I, K and M correspond to images taken from mice that were sacrificed 24 days post-ablation. Panels B, D, F, H, J, L and N correspond to images taken from mice that were sacrificed 40 days post-ablation. A) Olfactory marker protein (OMP) expression in the epithelium of 24 day ablated mice. No expression of OMP is observed in the epithelium on the ablated side (compare right half (arrow) with left half). B) OMP expression in the epithelium of 40 day ablated mice. Recovery of OMP expression has begun on the ablated side (right side), although this expression pattern is thinner and less robust than that of control (arrow). C) DAPI staining of bulbs from 24 day ablated mice shows significant reduction in glomerular size, but no apparent decrease in cell number. D) DAPI staining of bulbs from 40 day ablated mice show no dramatic differences compared to control, although glomerular size still appears somewhat reduced. E) Glutamate receptor subunit 1 (GluR1) expression in bulbs from 24 day ablated mice. Note stronger glomerular expression of GluR1 on the unablated side relative to the ablated side. However, GluR1 expression in the mitral layer was slightly but visibly stronger on the ablated side relative to control (arrows). F) GluR1 expression has returned in 40 day ablated animals to levels similar to control. G) Tyrosine hydroxylase (TH) expression in 24 day ablated animals is dramatically reduced (arrow). H) TH expression in 40 day ablated animals. Expression of TH has returned to levels similar to that of control. I) Connective tissue growth factor (CTGF) expression in 24 day ablated animals. On the unablated side, CTGF is expressed in both the mitral/tufted and glomerular layer. No expression in either layer is observed in the ablated animal (arrows). J) CTGF expression in 40 day ablated mice. Expression levels are slightly weaker than on the control side but is still clearly visible in mitral and glomerular layers. K) Protocadherin7 (Pcdh7) expression, like CTGF, is also detected in the mitral and glomerular layers in adult, although expression in the mitral layer is weak and inconsistent. On the ablated side, expression is reduced in both layers, but weak expression is still visible in some cells (arrows). L) Pcdh7 expression in 40 day ablated mice. Observed expression of Pcdh7 approximates that of the control bulb. M) Pcdh17 expression in 24 day ablated mice. Expression is present in both the mitral and glomerular layers. However, on the ablated side, only expression in the glomerular layer is affected (arrow). N) Pcdh17 expression in 40 day ablated mice. Expression has recovered and approximates that of control. Scale bar = 125 μm (7A-D); 200 μm (7E-N).