Figure 7

Double-label in situ hybridization with Tbr1. Nascent EPL/GL is dorsal and the granule layer is ventral in each section. Arrowheads in all panels indicate cells that express candidate genes but do not express Tbr1. A-C) Zinc finger csl-type containing 3 (Zcsl3) (red)/T-box brain gene 1 (Tbr1) (green). Note Zcsl3 expression extends beyond the Tbr1 expression domain. D-F) Protocadherin17 (red)/Tbr1 (green). G-I) Typical punctate pattern observed using a cDNA probe containing the common element (red)/Tbr1 (green)/DAPI (blue). J-L) Connective tissue growth factor (red)/Tbr1 (green). M-O) Gicerin (red)/Tbr1 (green). Scale bar = 100 μm.