Figure 3

Efficient nucleofection of single/multiple plasmids in mouse spinal cord NPCs. A: Strong expression of the green fluorescent protein (GFP) coded by the control pMax plasmid (2 μg) was observed in ~30% of the cells at 48 h after transfection. B: Time course of GFP expression revealed a fast but transient expression of the reporter gene. C: Cotransfection with pMax (2 μg) and RFP (2 μg). Due to a weaker expression, RFP could only be detected in a subpopulation of GFP expressing cells. However, almost all RFP expressing cells were GFP positive (95% ± 3) demonstrating efficient and reliable plasmid co-transfection coul be achieved by nucleofection.