Figure 2

(A) Schematic representation of GrlJ. The conserved domain database [51] and SMART [52] was used to predict conserved domains for the protein sequence and drawn to scale. Bmp, basic membrane protein; 7TMD, seven transmmembrane domain. (B) Hydropathy analysis of GrlJ. The putative transmembrane regions (amino acid residues 375–638) were used to deduce the hydrophobicity of GrlJ using the TMpred programme [53]. Positive scores indicate a membrane spanning potential which predicted the presence of 7 transmembrane regions (TM1, TM2, TM3, TM4, TM5, TM6, TM7). (C) Phylogenetic tree of Dictyostelium GrlJ and metabotropic GABA _B and glutamate receptors from other eukaryotes. A CLUSTALX alignment of the full length sequence of Dictyostelium GrlJ and selected GABA_B and glutamate receptors from other organisms was applied to the TreeView program to obtain a phylogenetic tree. The scale bar indicates 10% divergence. The proposed names were used for the Dictyostelium receptors and UniProt identifiers for the receptors from other organisms. C.e.: Caenorhabditis elegans; H.s.: Homo sapiens, D.m.: Drosophila melanogaster, G.c: Geodia cydonium. (D) Phylogenetic tree of the transmembrane regions of Dictyostelium GrlJ and metabotropic GABA _B receptors from other eukaryotes. Analysis of the transmembrane regions of the respective genes was carried out as described above applying the CLUSTALX and TreeView programme. Scale bar, 10% divergence.