Figure 5

NOGO-A expression during cell migration. A) H&E staining of optic tectum at HH30(E7) showing the generative zone (GZ), the migrating zone (MZ) and the first neuronal lamina (L1). B) Section in situ hybridization (sISH) of NOGO-A in an adjacent section demonstrating a corresponding radial pattern of expression in the region of migration. High magnification of the MZ (from boxed region in "A") shows the elongated processes of radial glia and associated migrating neurons. C) H&E for morphologic comparison; D) vimentin (Vim) immunohistochemistry (IHC) decorating the intermediate filaments of the radial glia; E) IHC of Neurofilament (NF) targeting migrating neurons; and F) sISH showing NOGO-A expression (red) more closely resembling NF staining. Scale bar 200 μm in A, B; 50 μm in C, D, E, F.