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Figure 4 | BMC Developmental Biology

Figure 4

From: Large-scale RNAi screens identify novel genes that interact with the C. elegans retinoblastoma pathway as well as splicing-related components with synMuv B activity

Figure 4

Inactivation of specific spliceosome components resembles synMuv B genes. (A) rsr-2 RNAi by feeding in WT (N2) animals results in extra intestinal nuclei (arrows) (this animal: 41 nuclei), as visualized by intestine specific expression of elt-2::GFP (B) lag-2::GFP reporter shows expression in distal tip cells and vulval cells (C) Ectopic expression of lag-2::GFP reporter in the intestine (arrows) and other cells (arrowheads) after lsm-2 RNAi. (D) Expression of scm::GFP in seam cells is not affected by lsm-4 RNAi. (E) lin-35 RNAi induced silencing of the scm::GFP transgene. (F) snr-3 RNAi by feeding in WT animal induced ectopic P granules in somatic cells (arrowheads), in addition to germ cell precursor (bottom). DAPI staining of DNA is in blue, staining of P granules detected with the K76 antibody in red. (G) Induction of ectopic vulval structures following snr-4 RNAi in lin-15A mutant larvae (L3 stage).

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BMC Developmental Biology

ISSN: 1471-213X

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