Figure 5
From: Regulation of Caenorhabditis elegans body size and male tail development by the novel gene lon-8
Northern blot analysis of lon-8 and lon-1 mRNA levels in Sma/Mab mutant strains. RNA was isolated from mixed populations of animals carrying ctIs40 [dbl-1xs] (which results in DBL-1 overexpression), dbl-1(nk3) or sma-6(e1482). Blots were hybridized with probes corresponding to lon-8, lon-1 or the loading control eft-2. Whereas lon-1 mRNA levels are clearly increased in dbl-1(nk3) and sma-6(e1482) animals, we could not detect a difference in lon-8 mRNA levels by microdosimetry in four experiments with independently prepared RNA.