Table 1 PCR Primer Sequences for Amplification of Ccm2, Map3k3, Map2k3, Map2k6, Map2k4, H2afz, and Luciferase
Gene product | Primer | Primer Sequence | Size (bp) | Ensembl Transcript ID |
|---|---|---|---|---|
Ccm2 | 5' | TCTGCTCAGTCTGTCTGCCTA | 371 | ENSMUST00000000388 |
| Â | 3' | CAAATATTGCTCGGTCCAGAA | Â | Â |
Map3k3 | 5' | CCATCCTTCAGGAAATCACAA | 374 | ENSMUST00000002044 |
| Â | 3' | CAATTGATGGGAGCACTAGGA | Â | Â |
Map2k3 | 5' | AGCCTATGGGGTGGTAGAGAA | 387 | ENSMUST00000019076 |
| Â | 3' | CCTTCCTTGTTGATGAGGACA | Â | Â |
Map2k6 | 5' | AGATGACCTGGAGCCGATAGT | 395 | ENSMUST00000019076 |
| Â | 3' | GCTTGACGTCTCGATGGATAA | Â | Â |
Map2k4 | 5' | TGGAGCTCATGTCTACCTCGT | 363 | ENSMUST00000046963 |
| Â | 3' | CGTACAATGTGATCCCCAAAC | Â | Â |
H2afz | 5' | CTCACCGCAGAGGTACTTGAG | 375 | ENSMUST00000041045 |
| Â | 3' | ATGCAGAAATTTGGTTGGTTG | Â | Â |
Luciferase | 5' | TTGACAAGGATGGATGGCTAC | 354 | N/A |
| Â | 3' | GTTTTCCGGTAAGACCTTTGC | Â | Â |