Figure 2

Detection of mRNA Transcripts encoding p38 MAPK Pathway Constituents and CCM2 during Mouse Preimplantation Development. RT-PCR products encoding Ccm2, Map3k3, Map2k3, Map2k6, and Map2k4 were detected in cDNA from one (1) embryo equivalent at timed stages of development 1C, 1-cell; 2C, 2-cell; 4C, 4-cell; 8C, 8-cell; M, morula; B, blastocyst; L, ladder (A). Kidney tissue RT positive controls (+) and no cDNA template negative controls (-) are shown. Representative image of three independent replicates. Quantitative analysis of Ccm2 (B), Map3k3 (C), Map2k3 (D), Map2k6, (E), Map2k4 (F) and Mapk14 (G). mRNA transcript levels in a developmental series of mouse preimplantation embryos by Real-Time RT-PCR. Data are normalized to Luciferase control (0.025 pg/embryo) and relative to 1-cell target gene mRNA levels. Relative mRNA levels are presented as the mean ± s.e.m. representative of three independent replicates. Bars with different letters represent significant differences in relative mRNA levels between embryo stages (P ≤ 0.05).