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Figure 1 | BMC Developmental Biology

Figure 1

From: Identification of the C. elegansanaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeast

Figure 1

Meiotic metaphase arrest induced by strong RNAi of B0511.9. Pairs of pictures show DIC and fluorescence images of embryos in the uterus of a mother carrying a GFP::H2B transgene marking nuclei. (A, B) wild-type embryos show progressively more nuclei as divisions proceed. (C, D) B0511.9(RNAi) embryos are all arrested at the one cell stage; staining of such embryos for beta-tubulin (red) and DNA (blue) shows arrest stage is at meiotic metaphase I (inset). (E, F) emb-27/Cdc16(RNAi) embryos arrested in metaphase of meiosis I [15], a phenotype identical to that of B0511.9(RNAi) in (C, D). Arrows in (D) and (F) point to sperm chromatin, indicating that the embryos have been fertilized.

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BMC Developmental Biology

ISSN: 1471-213X

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