Figure 1

The majority of the clonal offspring harbours different recipient oocyte-derived mitochondrial genotypes. An unrooted phylogenetic tree derived by maximum likelihood using TREE-PUZZLE 5.2 with default settings from an alignment of concatenated mtDNA sequences (3806 nucleotides: control region, MT-CO1, MT-CO3, and MT-ND4L) of the three donor cell lines, their SCNT clones (CA1 to CA7, CB1 to CB3, and CC1 and CC2) and of an European mitochondrial reference genome (GenBank:AF010406). The scale bar represents 0.001 substitutions per site, and quartet puzzling values are shown (all are >50). The numbers at the nodes (quartet puzzling values) indicate the frequencies of occurrence for 1,000 replicate trees. Quartet puzzling support values provide an estimate of support of a given branch and can be interpreted in much the same way as bootstrap values. CA5 is the most divergent donor A-derived clone which is highlighted by the ellipse (see below for amino acid changes).