Figure 4
From: Mechanism of G1 arrest in the Drosophilaeye imaginal disc
Factors contributing to G1 arrest. (A-B) PCNA-GFP expression (green) in discs with tkva12 (A) and smo3 tkva12 (B) clones. Upregulation in the posterior part of the NPR only occurs in the smo3 tkva12 clones. (C-D) Eye disc with smo3 (C) and tkva12 (D) clones (absence of GFP in red), stained for Dap antibody in green. The loss of smo, but not tkv, causes the loss of Dap in the furrow (arrows). (E) Eye disc with ato1 M+ clones (absence of green) showing no ectopic BrdU incorporation (red) in the NPR. (F) UAS-ato overexpression clones (marked by GFP in green) can inhibit CycB accumulation (red) close to the anterior part of the NPR (arrow in F').