Figure 8

Developmental expression of AmphiHtt in B. floridae larvae. In the whole mounts, anterior is on the left; cross sections are viewed from the posterior end of the animal. Scale lines for whole mounts = 50 μm and for cross-sections = 25 μm. A. Side view of 24-hour larva with AmphiHtt expression in the neural tube. B. Enlargement of the most anterior part of the preceding specimens showing conspicuous expression in the cerebral vesicle (cv) and anterior neural tube until the first pigment spot (arrow). Some positive neural cells (arrowhead) were also found just behind the first pigment spot. C-E. Consecutive cross-sections starting from c and proceeding along the dotted arrow shown in B. A positive signal is present in neurons of the cerebral vesicle. F,G. Cross-sections through levels f and g in B, showing a pair of specifically labelled ventrolateral neurons. H. Cross-section through the level shown by the arrowhead in B. AmphiHtt is expressed in a single ventrolateral neuron. I. Side view of 48-hour larva. AmphiHtt expression is only visible at the level of neural tube. J. High-resolution image of two-thirds of the preceding specimen. The AmphiHtt transcript is conspicuously present in the cerebral vesicle and neural tube. K-N. Cross-sections starting from k and proceeding along the dotted arrow shown in J. Labelled neurons were found in the developing frontal eye complex (K-M). Transcripts were also visible at the level of ventral infundibular cells (N). O, P. Cross-sections through the levels o and p shown in J. AmphiHtt is expressed in some ventrolateral neurons of the nerve cord. Abbreviations: n, notochord; cv, cerebral vesicle.