Figure 4

chch inhibition results in inappropriate cell movements. (A) A schematic representation of the transplantation scheme. ctMO or chch-ATGMO mRNA-injected donor cells were transplanted to the hemisphere of a sphere stage wild-type host. Embryos were photographed at 40% epiboly (B, F, J), germ ring (C, G, K) shield stage (D, H, L), and 24 hpf (E, I, M). The chch morphant cells (F-I) undergo greater spreading than the control cells and move toward the margin. At 24 hpf control cells are often found in anterior neural tissue (E) while the chch morphant cells are more frequently found in superficial cell layers of the trunk (I). The abnormal movement of chch morphant cells to superficial cell layers of the trunk is rescued by the injection of chch-mRNA (J-M).